AmpliSens® Pyroscreen PLASMO-screen AmpliSens® Pyroscreen PLASMO-screen

Cat. Number: PMQ-004-50-F-CE

Test for the detection of genetic polymorphisms in genes encoded the plasma factors of the coagulation system

AmpliSens^® Pyroscreen PLASMO-screen kit is an in vitro nucleic acid amplification test based on pyrosequencing technology for genotyping the single-nucleotide polymorphisms (SNPs).

AmpliSens^® Pyroscreen PLASMO-screen kit is intended for the detection of genetic polymorphisms in genes encoded the plasma factors of the coagulation system.

Polymorphisms for analysis

Locus	Product	Polymorphism	rs	Sequence to Analyze	Analysis	Genotype variants
F2	Prothrombin	20210 G>A	rs1799963	CTC/TGCTGAGA	reverse	GG GA AA
F5	Leiden Factor	R534Q G>A	rs6025	C/TGCCTGTCCA	reverse	GG GA AA
F7	Coagulation Factor VII	R353Q G>A	rs6046	CG/AGGGCACGTGG	forward	GG GA AA
FGB	Fibrinogen	455 G>A	rs1800790	C/TATTAAAATCAATAG	reverse	GG GA AA
SERPINE1	Plasminogen activator inhibitor	–675 5G>4G	rs1799768	GAGTCAGC	forward	5G/5G 5G/4G 4G/4G

Recommended extraction kits: RIBO-prep (K2-9-Et-100-CE), DNA-sorb-B (K1-2-100-CE). It is possible to use other nucleic acid extraction protocols.

Nesessary reagent kit for sample preparation PEERO-prep (K15-1611-40-CE).

NOTICE: This kit is compatible only with PyroMark Gold Q24 or PyroMark Gold Q96 MD instrument (QIAGEN, Germany).

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AmpliSens® Pyroscreen PLASMO-screen

PMQ-004-50-F-CE

Kit with reagents only for amplification step

All reagents are in individual vials (no PCR-tubes)

*Check the presence of a registration certificate.
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation

PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.

In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).