AmpliSens® Pyroscreen FOLATE-screen AmpliSens® Pyroscreen FOLATE-screen

Cat. Number: PMQ-002-50-F-CE

Test for the detection of genetic polymorphisms in genes associated with folate cycle

AmpliSens^® Pyroscreen FOLATE-screen kit is an in vitro nucleic acid amplification test based on pyrosequencing technology for genotyping single-nucleotide polymorphisms (SNPs).

AmpliSens^® Pyroscreen FOLATE-screen kit is intended for the detection of genetic polymorphisms in genes associated with folate cycle.

Polymorphisms for analysis

Locus	Product	Polymorphism	rs	Sequence to analyze	Analysis	Genotype
MTHFR	Methylenetetrahydro-folate reductase	A222V, C>T	rs1801133	AGC/TCGATTTC	forward	CC CT TT
MTHFR	Methylenetetrahydro-folate reductase	E429A, A>C	rs1801131	AGA/CAAGTGTCTT	forward	AA AC CC
MTR	Methionine synthase	D919G, A>G	rs1805087	A/GCCATTATGA	forward	AA AG GG
MTRR	Methionine synthase reductase	I22M, A>G	rs1801394	TA/GTGTGAGCA	forward	AA AG GG
SLC19A1	Folate carrier	H27R, A>G	rs1051266	GT/CGCCGCCA	reverse	AA AG GG

Recommended extraction kits: RIBO-prep (K2-9-Et-100-CE), DNA-sorb-B (K1-2-100-CE). It is possible to use other nucleic acid extraction protocols.

Nesessary reagent kit for sample preparation PEERO-prep (K15-1611-40-CE).

NOTICE: This kit is compatible only with PyroMark Gold Q24 or PyroMark Gold Q96 MD instrument (QIAGEN, Germany).

Информация для заказа

Наименование

Кат. №

Комплектация

AmpliSens® Pyroscreen FOLATE-screen

PMQ-002-50-F-CE

Kit with reagents only for amplification step

All reagents are in individual vials (no PCR-tubes)

*Check the presence of a registration certificate.
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation

PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.

In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).