+7 (495) 664-28-84AmpliSens® Pyroscreen IHD-screen AmpliSens® Pyroscreen IHD-screen
AmpliSens® Pyroscreen IHD-screen kit is an in vitro nucleic acid amplification test based on pyrosequencing technology for genotyping the single-nucleotide polymorphisms (SNPs).
AmpliSens® Pyroscreen IHD-screen kit is intended for detection of genetic polymorphisms in genes associated with susceptibility to ischemic heart disease.
Polymorphism for analysis
|
Locus |
Product |
Polymorphism |
rs |
Sequence to Analyze |
Analysis |
Variants |
|
AMPD1 |
Adenosine monophosphate deaminase 1 |
Q12X G>A |
rs17602729 |
AC/TGTGAGTA |
reverse |
GG GA AA |
|
CDKN2A/2B |
Cyclin-dependent kinase inhibitors |
G>C |
rs1333049 |
TC/GAACTGTTG |
reverse |
GG GC CC |
|
HIF1A |
Hypoxia-induced factor 1 alfa |
P582S C>T |
rs11549465 |
A/GTGACAACTG |
reverse |
CC CT TT |
|
MMP3 |
Matrix metalloproteinase 3 |
5А>6А |
rs3025058 |
TCCCCCCATCAAAGGA |
reverse |
5A/5A 5A/6A 6A/6A |
|
APOE |
Аpolipoprotein E |
C112R T>C |
rs429358 |
GT/CGCGGCCGCC |
forward |
TT TC CC |
|
APOE |
Аpolipoprotein E |
R158C C>T |
rs7412 |
C/TGCCTGGCAGT |
forward |
CC CT TT |
Recommended extraction kits: RIBO-prep (K2-9-Et-100-CE), DNA-sorb-B (K1-2-100-CE). It is possible to use other nucleic acid extraction protocols.
Nesessary reagent kit for sample preparation PEERO-prep (K15-1611-40-CE).
NOTICE: This kit is compatible only with PyroMark Gold Q24 or PyroMark Gold Q96 MD instrument (QIAGEN, Germany).
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation
PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.
In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).
