AmpliSens® Pyroscreen DIABETES-2-screen AmpliSens® Pyroscreen DIABETES-2-screen

Cat. Number: PMQ-015-50-F-CE

Test for the detection of genetic polymorphisms in genes associated with susceptibility to diabetes mellitus type 2

AmpliSens^® Pyroscreen DIABETES-2-screen kit is an in vitro nucleic acid amplification test based on pyrosequencing technology for genotyping the single-nucleotide mutations.

AmpliSens^® Pyroscreen DIABETES-2-screen kit is intended for the detection of genetic polymorphisms in genes associated with susceptibility to diabetes mellitus type 2.

Polymorphism for analysis

Locus	Product	Polymorphism	rs	Sequence to Analyze	Analysis	Genotype variants
KCNJ11	ATP-dependent potassium channels	K23E C>T	rs5219	GCCA/GAGCCCAGGT	reverse	CC CT TT
PPARG	PPARγ transcription factor	P12A C>G	rs1801282	G/CGTCAATAGGA	reverse	CC CG GG
TCF7L2	transcription factor 7	IVS3 C>T	rs7903146	C/TTATATAATTT	forward	CC CT TT
TCF7L2	transcription factor 7	IVS4 G>T	rs12255372	GTC/AATTCTTGCC	reverse	GG GT TT

Recommended extraction kits: RIBO-prep (K2-9-Et-100-CE), DNA-sorb-B (K1-2-100-CE). It is possible to use other nucleic acid extraction protocols.

Nesessary reagent kit for sample preparation PEERO-prep (K15-1611-40-CE).

NOTICE: This kit is compatible only with PyroMark Gold Q24 or PyroMark Gold Q96 MD instrument (QIAGEN, Germany).

Информация для заказа

Наименование

Кат. №

Комплектация

AmpliSens® Pyroscreen DIABETES-2-screen

PMQ-015-50-F-CE

Kit with reagents only for amplification step

All reagents are in individual vials (no PCR-tubes)

*Check the presence of a registration certificate.
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation

PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.

In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).