AmpliSens® Pyroscreen TONO-screen AmpliSens® Pyroscreen TONO-screen

Cat. Number: PMQ-004-50-F-CE

Test for the detection of genetic polymorphisms in genes associated with susceptibility to arterial hypertension

AmpliSens^® Pyroscreen TONO-screen kit is an in vitro nucleic acid amplification test based on pyrosequencing technology for genotyping the single-nucleotide polymorphisms.

AmpliSens^® Pyroscreen TONO-screen kit is intended for the detection of genetic polymorphisms in genes associated with susceptibility to arterial hypertension.

Polymorphisms for analysis

Locus	Product	Polymorphism	rs	Sequence to Analyze	Analysis	Genotype
ARDB2	β2 Adrenergic receptor	G16R G>A	rs1042713	G/AGAAGCCATGCG	forward	GG GA AA
AGT	Angiotensinogen	T207M C>T	rs4762	G/ATGGACAGCA	reverse	CC CT TT
AGT	Angiotensinogen	M268T T>C	rs699	A/GTCAGGGAGCA	reverse	TT TC CC
AGTR1	Angiotensin II type 1 receptor	A1666C A>C	rs5186	A/CTTAGCTACT	forward	AA AC CC
NOS3	Nitric oxide synthase	D298E G>T	rs1799983	GGGA/CTCATCTG	reverse	GG GT TT

Recommended extraction kits: RIBO-prep (K2-9-Et-100-CE), DNA-sorb-B (K1-2-100-CE). It is possible to use other nucleic acid extraction protocols.

Nesessary reagent kit for sample preparation PEERO-prep (K15-1611-40-CE).

NOTICE: This kit is compatible only with PyroMark Gold Q24 or PyroMark Gold Q96 MD instrument (QIAGEN, Germany).

Информация для заказа

Наименование

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Комплектация

AmpliSens® Pyroscreen TONO-screen

PMQ-004-50-F-CE

Kit with reagents only for amplification step

All reagents are in individual vials (no PCR-tubes)

*Check the presence of a registration certificate.
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation

PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.

In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).