AmpliSens® Pyroscreen TONO-screen AmpliSens® Pyroscreen TONO-screen

Cat. Number: PMQ-004-50-F-CE

Test for the detection of genetic polymorphisms in genes associated with susceptibility to arterial hypertension

AmpliSens® Pyroscreen TONO-screen kit is an in vitro nucleic acid amplification test based on pyrosequencing technology for genotyping the single-nucleotide polymorphisms.

AmpliSens® Pyroscreen TONO-screen kit is intended for the detection of genetic polymorphisms in genes associated with susceptibility to arterial hypertension.


Polymorphisms for analysis

Locus

Product

Polymorphism

rs

Sequence to Analyze

Analysis

Genotype

ARDB2

β2 Adrenergic receptor

G16R G>A

rs1042713

G/AGAAGCCATGCG

forward

GG

GA

AA

AGT

Angiotensinogen

T207M C>T

rs4762

G/ATGGACAGCA

reverse

CC

CT

TT

AGT

Angiotensinogen

M268T T>C

rs699

A/GTCAGGGAGCA

reverse

TT

TC

CC

AGTR1

Angiotensin II type 1 receptor

A1666C A>C

rs5186

A/CTTAGCTACT

forward

AA

AC

CC

NOS3

Nitric oxide synthase

D298E G>T

rs1799983

GGGA/CTCATCTG

reverse

GG

GT

TT

Recommended extraction kits: RIBO-prep (K2-9-Et-100-CE)DNA-sorb-B (K1-2-100-CE). It is possible to use other nucleic acid extraction protocols.

Nesessary reagent kit for sample preparation PEERO-prep (K15-1611-40-CE).

NOTICE: This kit is compatible only with PyroMark Gold Q24 or PyroMark Gold Q96 MD instrument (QIAGEN, Germany).

Информация для заказа
Наименование
Кат. №
Комплектация
AmpliSens® Pyroscreen TONO-screen
PMQ-004-50-F-CE
Pyrosequencing Kit with reagents only for amplification step All reagents are in individual vials (no PCR-tubes)
*Check the presence of a registration certificate.
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation

PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.

In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).

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