AmpliSens® Pyroscreen BRCA-screen AmpliSens® Pyroscreen BRCA-screen

Cat. Number: PMQ-005-50-F-CE

Test for the detection of mutations in BRCA1 and BRCA2 genes associated with higher risk of breast and/or ovarian cancer

AmpliSens® Pyroscreen BRCA-screen kit is an in vitro nucleic acid amplification test based on pyrosequencing technology for genotyping the single-nucleotide mutations.

AmpliSens® Pyroscreen BRCA-screen kit is intended for the detection of mutations in BRCA1 and BRCA2 genes associated with higher risk of breast and/or ovarian cancer.

Mutations for analysis

Locus	Product	Mutation	rs	Sequence to Analyze	Analysis	Risk allele
BRCA1	Breast cancer gene 1	185delAG	rs80357713	T[CT]AAGATTTTCTGC	reverse	delAG
BRCA1	Breast cancer gene 1	300T>G (C61G)	rs28897672	T/GGTCCTTTATGTA	forward	G
BRCA1	Breast cancer gene 1	2080delA	rs80357522	[T]CTTTATCTCT	reverse	delA
BRCA1	Breast cancer gene 1	4153delA	rs80357711	GA[A]GAAAGAGGA	forward	delA
BRCA1	Breast cancer gene 1	5382insC	rs80357906	[G]ATTCTCTTG	reverse	insC
BRCA2	Breast cancer gene 2	6174delT	rs80359550	[A]CTTGCTGTG	reverse	delT

Recommended extraction kits: RIBO-prep (K2-9-Et-100-CE), DNA-sorb-B (K1-2-100-CE). It is possible to use other nucleic acid extraction protocols.

Nesessary reagent kit for sample preparation PEERO-prep (K15-1611-40-CE).

NOTICE: This kit is compatible only with PyroMark Gold Q24 or PyroMark Gold Q96 MD instrument (QIAGEN, Germany).

Информация для заказа

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AmpliSens® Pyroscreen BRCA-screen

PMQ-005-50-F-CE

Kit with reagents only for amplification step

All reagents are in individual vials (no PCR-tubes)

*Check the presence of a registration certificate.
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation

PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.

In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).