AmpliSens® Florocenosis/Bacterial vaginosis-FRT AmpliSens® Florocenosis/Bacterial vaginosis-FRT

Cat. Number: R-B74-100-FT(RG)-CE

Real-time PCR kit for quantitative detection of Gardnerella vaginalis, Atopobium vaginae, Lactobacillus spp., and total quantity of bacteria

AmpliSens® Florocenosis / Bacterial vaginosis-FRT PCR kit is an in vitro nucleic acid amplification test for diagnosing bacterial vaginosis (quantitation of DNA of Gardnerella vaginalis, Atopobium vaginae, Lactobacillus spp., and total amount of bacteria) in the clinical material using real-time hybridization-fluorescence detection.

This PCR kit allows assessment of the ratio between the total amount of bacteria, lactobacteria, and opportunistic pathogenic bacteria associated with bacterial vaginosis (Gardnerella vaginalis, Atopobium vaginae) in the vaginal biotope.

Recommended extraction kit: DNA-sorb-AM (K1-12-100-CE). It is possible to use other nucleic acid extraction protocols.


Adapted for:

  • Rotor-Gene 3000/6000 (Corbett Research, Australia)
  • Rotor-Gene Q (QIAGEN, Germany)
  • iCycler iQ, iCycler iQ5 (Bio-Rad, USA)

4 or more optical channels are required

Информация для заказа
Наименование
Кат. №
Комплектация
AmpliSens® Florocenosis/Bacterial vaginosis-FRT
R-B74-100-FT(RG)-CE
FRT Kit with reagents only for amplification step All reagents are in individual vials (no PCR-tubes) + Taq-F polymerase (“hot start”)
*Check the presence of a registration certificate.
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation

PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.

In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).

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