AmpliSens® ARVI-screen-FRT

Cat. Number: R-V57-100-F(RG,iQ,Dt)-CE

Real Time PCR Kit for  multiplex detection and identification of specific nucleic acid fragments of pathogens that cause acute respiratory viral infections – human Respiratory Syncytial virus (hRSv) RNA; human Metapneumovirus (hMpv) RNA; human Parainfluenza virus-1-4 (hPiv) RNA; ОС43, Е229, NL63, and HKUI human Coronavirus (hCov) RNA; human Rhinovirus (hRv) RNA; human B, C, and E Adenovirus (hAdv) DNA; and human Bocavirus (hBov) DNA – in the clinical material

AmpliSens® ARVI-screen-FRT PCR kit is an in vitro nucleic acid amplification test for multiplex detection and identification of specific nucleic acid fragments of pathogens that cause acute respiratory viral infections – human Respiratory Syncytial virus (hRSv) RNA; human Metapneumovirus (hMpv) RNA; human Parainfluenza virus-1-4 (hPiv) RNA; ОС43, Е229, NL63, and HKUI human Coronavirus (hCov) RNA; human Rhinovirus (hRv) RNA; human B, C, and E Adenovirus (hAdv) DNA; and human Bocavirus (hBov) DNA – in the clinical material (nasal and oropharyngeal swabs, sputum, aspirate of trachea, bronchoalveolar lavage, bronchial washing fluid, and autopsy material) by using real-time hybridization-fluorescence detection of amplified products.

NOTICE: This kit requires reverse transcription step. We recommend Reverta-L (К3-4-50-CE) reverse transcription kit for better results.

Recommended extraction kits: RIBO-sorb (K2-1-Et-100-CE), RIBO-prep (K2-9-Et-100-CE). It is possible to use other nucleic acid extraction protocols.

Adapted for:

  • Rotor-Gene 3000, Rotor-Gene 6000 (Corbett Research, Australia)
  • Rotor-Gene Q (QIAGEN, Germany)
  • iCycler iQ or iCycler iQ5 (Bio-Rad, USA)
Информация для заказа
Наименование
Кат. №
Комплектация
AmpliSens® ARVI-screen-FRT
R-V57-100-F(RG,iQ,Dt)-CE
FRT Kit with reagents only for amplification step All reagents are in individual vials (no PCR-tubes) + Taq-F polymerase (“hot start”)
*Check the presence of a registration certificate.
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation

PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.

In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).

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