+7 (495) 664-28-84AmpliSens® MTC-diff-FRT
AmpliSens® MTC-diff-FRT PCR kit is an in vitro nucleic acid amplification test for differentiation of the DNA of Mycobacterium tuberculosis complex (MTC) including the human (M.tuberculosis), the bovine (M.bovis) and also the vaccine (M.bovis BCG) strains in the clinical material and microorganism cultures using real-time fluorescence hybridization detection of amplified products.
Recommended extraction kits: RIBO-prep (K2-9-Et-100-CE), DNA-sorb-В (K1-2-100-CE), DNA-sorb-C (K1-6-50-CE). It is possible to use other nucleic acid extraction protocols.
Adapted for:
- Rotor-Gene 3000, Rotor-Gene 6000 (Corbett Research, Australia)
- Rotor-Gene Q (QIAGEN, Germany)
- iCycler iQ or iCycler iQ5 (Bio-Rad, USA)
- SmartCycler II (Cepheid, USA)
- Mx 3000P, Mx3005 (Stratagene, USA)
NOTICE: AmpliSens® MTC-diff-FRT PCR kit is intended as a supplement test for AmpliSens® MTC-FRT PCR kit and is used for analysis of the DNA previously extracted from the clinical material. The Internal Control STI-87 (IC) used for this experiment is also taken from AmpliSens® MTC-FRT PCR kit, thus consult the AmpliSens® MTC-FRT PCR kit Instructions Manual for further information.
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation
PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.
In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).
