+7 (495) 664-28-84AmpliSens® Corynebacterium diphtheriae / tox-genes-FRT PCR kit AmpliSens® Corynebacterium diphtheriae / tox-genes-FRT PCR kit
AmpliSens® Corynebacterium diphtheriae / tox-genes-FRT PCR kit is an in vitro nucleic acid amplification test for qualitative detection of Corynebacterium diphtheriae DNA and genes encoding toxins of Corynebacterium diphtheriae and Corynebacterium ulcerans in the biological material (nasopharyngeal swabs, oropharyngeal swabs, swabs from disease sites, germ culture), using real-time hybridization-fluorescence detection of amplified products.
The biological material for study may be taken from patients suspected of diphtheria etiology of the disease (laryngotracheitis, laryngitis, croup); from patients with tonsillitis with a pathological effusion on the tonsils, with suspicion of the pharyngeal (paratonsillar) abscess; infectious mononucleosis; stenosing laryngotracheitis; from persons who were in contact with the source of the infection (for epidemiological reasons); from persons newly admitted to specialized long-stay institutions for children and adults (for prophylactic purposes).
Recommended extraction kit: RIBO-prep (K2-9-Et-100-CE). It is possible to use other nucleic acid extraction protocols.
Adapted for:
- Rotor-Gene 3000, Rotor-Gene 6000 (Corbett Research, Australia);
- Rotor-Gene Q (QIAGEN, Germany);
- iCycler iQ5 (Bio-Rad, USA);
- CFX96 (Bio-Rad, USA)
4 or more optical channels are required
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation
PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.
In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).
