AmpliSens® listeria monocytogene screen-titre-FRT

Cat. Number: H-2161-1-1-CE

Real Time PCR Kit for detection and quantitation of DNA of Listeria monocytogenes in the biological material

AmpliSens® Listeria monocytogenes-screen/monitor-FRT PCR kit is an in vitro nucleic acid amplification test for detection and quantitation of DNA of L. monocytogenes in the biological material (whole blood, umbilical cord blood, cerebrospinal fluid (CSF), node aspirates, swabs from the respiratory tract, swabs from the eye conjunctiva, amniotic fluid, placenta, swabs from vagina mucous membrane, urine, breast milk, meconium, feces, autopsy material, environmental samples (concentrated water samples (wastewater, potable water, surface water)), liquid medium for primary enrichment of food product) using real-time hybridization-fluorescence detection of amplified products.

Recommended extraction kits: RIBO-prep (K2-9-Et-100-CE). It is possible to use other nucleic acid extraction protocols.

Adapted for:

  • Rotor-Gene Q (QIAGEN, Germany)
  • CFX 96 (Bio-Rad, USA)
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Кат. №
Комплектация
AmpliSens® Listeria monocytogenes-screen/monitor –FRT
H-2161-1-1-CE
FRT Kit with reagents only for amplification step All reagents are in individual vials (no PCR-tubes) + Taq-F polymerase (“hot start”)
*Check the presence of a registration certificate.
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation

PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.

In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).

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