+7 (495) 664-28-84AmpliSens Yersinia enterocolitica/ Y.pseudotuberculosis -FRT PCR kit
Cat. Number: R-B64(RG,iQ)-CE
Real time PCR Kitfor qualitative detection and differentiation of DNA of virulent and avirulent Yersinia enterocolitica strains (virulence is assessed by genes encoding enterotoxin (Yst), attachment invasion locus (ail), and plasmid pYV adhesion (yadA)) and Yersinia pseudotuberculosis strains in environmental samples and clinical material
AmpliSens® Yersinia enterocolitica / Y.pseudotuberculosis-FRT PCR kit is an in vitro nucleic acid amplification test for qualitative detection and differentiation of DNA of virulent and avirulent Yersinia enterocolitica strains (virulence is assessed by genes encoding enterotoxin (Yst), attachment invasion locus (ail), and plasmid pYV adhesion (yadA)) and Yersinia pseudotuberculosis strains in environmental samples (concentrated water samples) and clinical material (feces) using real-time hybridization-fluorescence detection of amplified products.
Recommended extraction kits: RIBO-prep (K2-9-Et-100-CE), RIBO-sorb (K2-1-Et-100-CE), DNA-sorb-B (K1-2-100-CE). It is possible to use other nucleic acid extraction protocols.
Adapted for:
- Rotor-Gene 3000, Rotor-Gene 6000 (Corbett Research, Australia)
- iCycler iQ or iCycler iQ5 (Bio-Rad, USA)
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation
PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.
In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).
