AmpliSens® HCV-genotype-FRT AmpliSens® HCV-genotype-FRT

Cat. Number: R-V1-G(1-4)-2x(RG,iQ,Mx,Dt,SC)-CE

Real Time PCR kit for qualitative detection and differentiation of hepatitis C virus (HCV) genotypes 1a, 1b, 2, 3a, and 4

AmpliSens^® HCV-genotype-FRT PCR kit is an in vitro nucleic acid amplification test for qualitative detection and differentiation of hepatitis C virus (HCV) 1a, 1b, 2, 3a and 4 genotypes in the biological material (peripheral blood plasma) using real-time hybridization-fluorescence detection of amplified products.

AmpliSens^® HCV-genotype-FRT PCR kit is recommended for use after detection of hepatitis C virus RNA by qualitative or quantitative analysis with the use of PCR kits manufactured by FBIS CRIE (for example, AmpliSens^® HCV-FRT (real-time hybridization-fluorescence detection).

NOTICE: This kit requires reverse transcription step. We recommend Reverta-L (К3-4-50-CE) reverse transcription kit for better results.

Recommended extraction kits: RIBO-sorb (K2-1-Et-50-CE) or RIBO-prep (K2-9-Et-50-CE). It is possible to use other nucleic acid extraction protocols.

Adapted for:

Rotor-Gene 3000 or Rotor-Gene 6000 (Corbett Research, Australia)
Rotor-Gene Q (QIAGEN, Germany)
iCycler iQ5 (Bio-Rad, USA)
SmartCycler II (Cepheid, USA)
Mx3000P, Mx3005P (Stratagene, USA)

2 optical channels are required

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AmpliSens® HCV-genotype-FRT

R-V1-G(1-4)-2x(RG,iQ,Mx,Dt,SC)-CE

Kit with reagents only for amplification step

All reagents are in individual vials (no PCR-tubes) + Taq-F polymerase (“hot start”)

*Check the presence of a registration certificate.
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation

PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.

In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).