+7 (495) 664-28-84AmpliSens® WNV-FRT
AmpliSens® WNV-FRT PCR kit is an in vitro nucleic acid amplification test for qualitative detection of West Nile virus (WNV) RNA in the clinical material (blood plasma, serum, leukocytic fraction of blood, cerebrospinal fluid, and urine) and human autopsy material (brain, liver, spleen, and lymph node tissue), animal material (brain tissue), mosquitoes and ticks using real-time hybridization-fluorescence detection of amplified products.
The material for RT-PCR is RNA-samples extracted from test material. Reverse transcription step is included.
Recommended extraction kits: RIBO-sorb (K2-1-Et-100-CE), RIBO-prep (K2-9-Et-100-CE), RIBO-zol-C (K2-13-100-CE), MAGNO-sorb (K2-16-1000-CE). It is possible to use other nucleic acid extraction protocols.
Adapted for:
- Rotor-Gene 3000, Rotor-Gene 6000 (Corbett Research, Australia)
- Rotor-Gene Q (QIAGEN, Germany)
- iCycler iQ5 (Bio-Rad, USA)
- Mx3000P (Stratagene, USA)
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation
PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.
In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).
