AmpliSens® FiloA-screen-FRT PCR kit AmpliSens® FiloA-screen-FRT PCR kit

Cat. Number: H-2781-1-4-CE

Real-time PCR kit for qualitative detection of RNA of Ebola virus variant Zaire (EBOV Zaire), Ebola virus variant Sudane (SUDV) and Marburg virus (MARV) of Filoviridae family in the human biological material

AmpliSens® FiloA-screen-FRT PCR kit is an in vitro nucleic acid amplification test for qualitative detection of RNA of Ebola virus variant Zaire (EBOV Zaire), Ebola virus variant Sudane (SUDV) and Marburg virus (MARV) of Filoviridae family in the human biological material (whole blood or its components: leucocytes and plasma), using real-time hybridization-fluorescence detection of amplified products.

This PCR kit is used for the analysis of biological material, taken from the persons suspected of filoviral haemorrhagic fevers without distinction of form and presence of manifestation. The material for RT-PCR is RNA-samples extracted from test material. Reverse transcription step is included.

Recommended extraction kits: RIBO-prep (K2-9-Et-100-CE), RIBO-zol-B (K2-3-100-CE). It is possible to use other nucleic acid extraction protocols.

Adapted for:

  • Rotor-Gene 3000/6000 (Corbett Research, Australia)
  • Rotor-Gene Q (QIAGEN, Germany)

Информация для заказа
Наименование
Кат. №
Комплектация
AmpliSens® FiloA-screen-FRT PCR kit
H-2781-1-4-CE
FRT Kit with reagents only for amplification step All reagents are in individual vials (no PCR-tubes) + Taq-F polymerase (“hot start”)
*Check the presence of a registration certificate.
In its absence, the product is not intended for medical purposes in the territory of the Russian Federation.
**RUO- the product is not intended for medical purposes in the territory of the Russian Federation

PCR is the basic method when working with nucleic acids, allows you to multiply the number of copies of the target DNA site and determine the initial concentration of the desired sequences during real-time analysis time.

In addition, this method has been widely used in molecular diagnostics for the determination of pathogens infectious diseases and predispositions to hereditary diseases. Cooling and heating of samples with the necessary frequency and duration required for conducting PCR, provided by special devices – amplifiers (thermal cyclers).

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